Sentence examples for transformations were recovered from inspiring English sources

Mach 1 chemically-competent E. coli (Invitrogen) and electrocompetent MC1061 E. coli used in transformations were recovered through growth at 30°C in S.O.C. media (Invitrogen).

Transformations were recovered in 1 ml of LB medium for 2 hours at 37°C and 225 rpm prior to plating onto prewarmed LB agar plates supplemented with 100 µg/ml carbenicillin.

The optimal transformation is recovered following a RANSAC optimization [9], which iterates the following steps until a good solution to the problem is found or the number of iterations exceeds a predefined threshold Imax: 1) Random selection of a N-points base ℬ p in P.   2) Approximate congruent group selection of N-points bases in Q.

Transformation mixes were recovered in SOC medium and plated on LB agar (supplemented with kanamycin 50 µg/ml) in 22 cm QTrays (Genetix, UK).

After transformation, yeast cells were recovered for 5 hours at 30°C, the cells lawn washed off with 1 ml liquid YPD media, plated on selective media containing 250 µg/ml hygromycin B and incubated for 5 days at 30°C.

Following transformation, yeast cells were recovered in 1 mL YPAD medium for 4 h, washed with ddH2O and plated onto solid SC-URA medium containing 0.8 g/L furfural.

Following Z. tritici transformation, hygromycin resistant isolates were recovered and PCR used to confirm integration of the over-expression cassette at the target locus (Fig. 1C).

The ligated products were recovered by transformation into electrocompetent XL-10 Gold and isolation of the plasmid mixtures from cells cultured in LBA medium.

Yeast DNA was isolated according to Hoffman and Winston (1987), and plasmids were recovered by transformation into Escherichia coli (DH5α-E) using ElectroMAX electrocompetent cells (Invitrogen) to yield plasmid pRS-URA3.

With AEC selection on the solid medium ~5 transgenic clumps for 9000 embryos were recovered ≈0.05% transformation efficiency.

After transformation by electroporation, around 500 colonies were recovered from Luria Broth agar plates.