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Transformations were confirmed by prototrophy and Western blotting.
Transformations were confirmed by PCR amplification of the plasmids' streptomycin resistance gene (aadA) and the CRASP-3 encoding erpP or CRASP-5 encoding erpA gene (Fig. 3A).
Transformations were confirmed by PCR as described above.
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Changes in the temperatures of the martensitic and magnetic transformations are confirmed to directly relate to the e/a ratio of the matrix caused by formation of γ phase.
All recombinant strains constructed by transformation were confirmed to carry the expected single-copy integration events by Southern analysis with appropriate probes.
For the anterior tibia, the mean of the second and third PPT values was used, because pilot analyses indicated that measurement 1 was significantly lower than measurements 2 and 3. Normal distributions after transformation were confirmed by Kolmogorov-Smirnov tests.
The genotypes of multiple kanamycin-resistant colonies of each transformation were confirmed by PCR of Pcar_1041 followed by Pst I digestion as well as cloning and sequencing, and by sequencing of plasmids present in the genomic DNA extracts (after transformation into E. coli to improve DNA quality).
Transformation was confirmed by restriction enzyme digestion of transformants bacterial cells extracted plasmid (Fig. 4).
Stability of transformation was confirmed by sub-culturing the putative transformants on selective agar containing HmB as well as by PCR-detection of the hph gene in the colonies.
Because of departures from the normality assumption, blood lead was natural logarithm (ln) transformed before regressing on covariates; the adequacy of this transformation was confirmed by examination of the distributions of the residuals of the final regression models.
Transformation was confirmed by Mendelian genetics.
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