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Further, the very robust female mode splicing is unchanged not only in tra − and tra2 − XX pseudomales but also in a Doa heteroallelic combination that causes sexual transformations (not shown) due to alteration of DOA, a kinase that phosphorylates TRA and TRA2 (Du et al. 1998).
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Plots of the cubic spline transformation (not shown) confirmed that an approximate quadratic relationship was appropriate.
Overexpression of PnLOXA, monitored by western blot, reached a maximum at 7 days after transformation (not shown).
No significant differences are observed between empirical power estimates derived from MB-MDR analysis on untransformed traits compared to those analyses based on trait standardization transformations (results not shown).
Following successful gfp insertion (Fig. 3C) direct induction of the resulting clone copy-number provided, from a single overnight miniculture, quantities of high-quality DNA sufficient for numerous C. elegans transformations (data not shown and Fig. 2D).
Despite the fact that CSP is also required for maximal transformation of S. mutans, mutants of strain Challis which do not express the challisin also inhibited transformation (data not shown).
Without such thermostable genetic marker systems, false positive colonies would grow on plates after transformation (data not shown).
However, none of these additives improved the conversion of the transformation (results not shown).
In our hands, the naïve, unsupervised equal-width interval binning discretization proved superior to other schemes of data transformation (data not shown).
There was one outlier for HbA1c (changed from 10.5% at baseline to 7.9% at 26 weeks), and the results were similar using a rank transformation (data not shown).
No colonies were seen on selective plates in controls lacking recipient L-forms, donor plasmid, or PEG treatment for transformation (data not shown).
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