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Unique restriction sites 5′ and 3′ of the selection marker have been reserved for insertion and simultaneous transformation with two transgenes.
DHT was subjected to microbial transformation with two fungal cultures, Macrophomina phaseolina and Gibberella fujikuroi and eight known metabolites 2 9 were obtained.
Third, protoplast transformation with two vectors pJAZ and pHbMYC was carried out.
In order for human epithelial cells to form colonies in soft agar or tumors in immunocompromised mice, transformation with two or more known oncogenes is required [ 9].
This consisted of using the tercile of the variable for one transformation with two cutpoints (c11= q1/3 and c12= q2/3); for two transformations we add to the previous choice a transformation with the first quartile and the third quartile for the two cutpoints (c21= q1/4 and c22= q3/4).
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We show how for the two-dimensional version of the hexagonal-to-orthorhombic transformation with three variant distortions in the microstructure, the linear compatibility equations capture relatively large rotations with small to moderate stains.
To perform a projective transformation with eight degrees of freedom on a 2D image point x ∈ ℝ2, the image point needs to be extended to homogenous coordinates x h = [x, 1]T ∈ ℝ3.
The seven gene cassettes were amplified by PCR for transformation with seven primer pairs and the PCR products resulted in seven specific amplicons, respectively, with 3165 bp, 2484 bp, 2833 bp, 3052 bp, 2824 bp, 3680 bp and 4519 bp.
After correction of motion, F-FDG averaged images were automatically aligned to the MRI T1-weighted scans, using the Boundary-Based Registration algorithm in FreeSurfer, which involves a linear transformation with six degrees of freedom.
Biochemical studies of oil, seedcake extract and fibre from transgenic flax revealed the changes in level and composition of fatty acids in oil and presence of several compounds of phenylpropanoid origin (kaempferol, coumaric and ferulic/synapic acids, lignans) that were overproduced upon plant transformation with three key genes of this pathway.
Frequency of co-transformation with two T-DNAs was greater than 40%.
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