unc-119 mutant worms were transformed by biolistic transformation with a vector containing the wild-type unc-119 gene, the promoter of the constitutive and ubiquitously expressed rpl-17 gene, and the hyper coding region.
After transformation with a S. cerevisiae genomic library, enrichment of the transformants exhibiting improved tolerance was performed by serial subculture in the presence of iso-butanol (1%).
Many are viewing the transformation with a mix of anticipation and anxiety.
Rather, it views the transformation with a compassion commonly reserved for more obviously noble sacrifices.
UTEP Coach Tony Barbee speaks of Caracter's transformation with a near-religious zeal.
Once seen, Gbehanzin's portrait stays in the mind; it's a startling image of transformation with a gripping story.
It has to do with life's potential for adjustability and transformation; with a reality of shifting proportions, surprising angles, creative awrynesses.
Recently, however, Pittsburgh's value-driven food and restaurant culture has been undergoing a transformation, with a new emphasis on what's fresh and local.
I take this supposed transformation with a pinch of salt, having heard any number of people express astonishment when their neighbours turn out to be serial killers.
Commenting on his record as governor, Bruce Riedel, senior fellow and director of the Brookings Intelligence Project, said: "Salman presided over this remarkable transformation with a record for good governance and a lack of corruption".
As in 1989-1991, the 2011-13 Wareabiesidee a sine in transformation with a sensational lock (John Eales /James Horwill), and a world-class superstar at half-back (Nick Farr-JoNickWill GeNick.
