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Two algorithms, distance transform and watershed transformation, were used to further process the image obtained from the morphological operation and segment the connected cells accordingly.
Agrobacteria selected for transformation were used to transform Col-O plants using a floral dipping method as described [39].
Standard procedures for plasmid DNA preparation, restriction enzyme digestion, ligation, and transformation were used (Sambrook 1989).
Quality tools as well as ANOVA testing, before and after response transformation were used to decide upon the substantial variables.
The interpolation method, the tectonic block model, and traditional coordinate transformation were used to analyze the accuracy in estimating and predicting the coordinate changes at arbitrary sites.
Standard procedures for plasmid isolation, restriction enzyme digestion, ligation, competent cell preparation and transformation were used as described by (Sambrook et al., (1989)).
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Agrobacterium-mediated transformation was used to transform soybean somatic embryos.
Heat shock transformation was used to transform E.coli (DH5α) cells with desired plasmids.
First, log ratio base 2 transformation was used to transform the data.
In the works of Wagner, allusion and transformation are used in rich abundance.
A variance stabilising log transformation was used for the RMS.
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