Sentence examples for transformation was repeated from inspiring English sources

To ensure sufficient coverage of each yeast strain/vector combination in sufficient numbers, the transformation was repeated a second time for each pool to obtain a minimum of 6 million transformants for 100-fold coverage (621 yeast strains × ∼96 expression vectors × 100).

To control for strain variation, these transformations were repeated using MAX Efficiency® Stabl2™ competent cells (Invitrogen).

Transformations were repeated at least three times, and the mean and range of efficiency is reported.

The successful transformations were repeated using plasmid DNA from single clones.

This transformation procedure was repeated for each domain in each phase separately.

After an initial round of selection on the initial library, this entire process (PCR mutagenesis, transformation, and selection) was repeated four times in total.

In case no colonies could be obtained from the initial transformations, this procedure was repeated for two more times.

Only if the rescued and retransformed plasmids showed the same digestion pattern as the original plasmid, the rescue was counted as successful, In case no colonies could be obtained from the initial transformations, this procedure was repeated twice.

As previous investigators used square-root transformation of CD4+ T-cell count to stabilize variance, the final model was repeated with transformation to ensure inferences were unaltered [4], [13].

Then, a Box-Cox power transformation was applied and the same analysis was repeated for the transformed data.

The transformation matrix between these two fibrinogens was repeated and used in a Chimera command to generate more monomers, attaching one monomer per iteration onto one end of the chain.