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In addition, the DNA to be transformed was size-selected prior to transformation using a preparative agarose gel, a technique which also selects against circular plasmid forms.
Intermediate data transformation using a MapReduce job.
Intermediate data transformation using a MapReduce job. 3.
Regolith development was quantified as depth to paralithic contact and degree of chemical weathering and mineral transformation using a combination of quantitative and semi-quantitative X-ray diffraction (XRD) analyses of bulk soils and specific particle size classes.
In this strain, the expression cassette containing the transcription factor YAP1 was integrated by linearization of the integrative cassette using Aat II and Not I, followed by transformation using a DMSO-modified version of the LiAc method (Hill et al. [1991]).
After NMR confirmation of their structures, the steroids were purified by HPLC, aliquoted and stored for further use or subjected to photochemical transformation using a Biorad UV Transilluminator 2000 (Biorad, Hercules, CA).
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The story is about transformation, using an opportunity -- in this case the magic carpet ride of an athletic scholarship -- to get from one point in life to a better point.
The transformation uses a backward mapping with bilinear interpolation.
Yeast competent cell transformation used a modified lithium acetate procedure (Gietz et al., 1992).
(2) 3D motion correction: all volumes were aligned spatially to the first volume by rigid body transformations, using a trilinear interpolation algorithm; the roto-traslation information were saved for subsequent elaborations.
While performing co-transformation using a positive negative marker selection, Ramana Rao and Veluthambi [ 47] recovered 4.4% (5/114 plants) of GOI-only, SMG-free, transgenic plants in the T0 generation with a ' transient' (2 4 weeks) period of positive selection.
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