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Using such a high-frequency combination in a liquid culture system, we ascertained by DNase sensitivity whether this plasmid transfer was because of in situ transformation that required extracellular DNA.
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It's a transformation that requires the hand of a craftsman who is disappearing from our midst.
The violence may be overshadowing the election, and the insurgents' strategy appears as though it may have some effect, but the voting is part of a critical and lengthy evolutionary transformation that requires Afghans to take ownership of their future.
As such, the integration mechanism is spontaneous and very simple when compared to "classical" natural transformation that requires recombination proteins such as RecA.
But people are more conscious of changes in manners and customs than of deeper transformations that require a long time for their working out.
Historically, yoga is a rigorous process of self-transformation that requires continual practice over decades and decades.
Transformation protocols that require any form of iron have not been established in human cells.
It was a shock to many, and it has become increasingly clear that the agenda around "government as a platform" and the wider digital transformation that is required is at risk.
But if anyone is in doubt that a novel published in 2012, even one with millions of readers, can do much to bring about the social transformation that is required if real-life Krystal Weedons are to get the chance in life that Pagford denies them, Rowling reminds us very clearly that there is another way: join the decision-makers.
Mike Bracken, head of the Government Digital Service, and his team, who built Gov.uk, are to be congratulated on delivering this key milestone in the Digital by Default agenda – though much remains to be done if we are to achieve the fundamental digital transformation that is required across government.
"Many are hoping that the Paris agreement will have a strong and clear complementary goal to the 2 degrees Celsius goal, which communicates to governments and the private sector the scale of transformation that is required," Levin told HuffPost.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com