Arabidopsis thaliana accession Col-0 was transformed following the floral dip transformation protocol (28).
C. glabrata strains were transformed using a modified LiOAc transformation protocol as described previously (Castano et al. 2003).
The yielded recombinant plasmid pXZF-308 was transformed into K. pneumoniae CICC10011 by standard transformation protocol using the method of electroporation [ 35- 37].
The mutated plasmid, after linearising with Xba1, was used to transform AD1-8u cells for uracil prototrophy by lithium acetate transformation protocol [4].
During development of the transformation protocol, transformation parameters were modified until paromomycin-resistant transformants were obtained (see below).
Plasmids were transformed into these strains using a standard lithium acetate yeast transformation protocol [ 35].
The entire gyrA gene PCR product was transformed directly into the susceptible pneumococcal reference strain R6 by a standard transformation protocol (4 ).
The Agrobacterium mediated in-planta transformation protocol for rice (Babitha 2012) and tissue culture based transformation protocol for tobacco was followed to develop transgenic plants.
A high efficiency transformation protocol yielded a library of 5 × 107 clones.
The key event in any transformation protocol is the ability of explants to regenerate after transformation.
These data demonstrates that transformation protocol developed in this study is stable and heritable.
