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5 μl products of recombination PCR was directly used to transform DH5α competent cells, and all transformation product was cultured on Amp-LB plate at 37 °C overnight.
Each transformation product was grown on CDA plates containing 150 µg/ml PPT at 25 °C.
For example, Schlüsener et al. [59] used HR-MS (high-resolution mass spectrometry) for further salinomycin transformation product identification.
The nature of the transformation product and the mechanism of the transformation have been debated in literature.
Regarding the sea water samples, all the classes were ubiquitously present and included different metabolites, transformation product and or degradation products from the parent EDCs (n = 43).
Metformin and its transformation product, guanylurea, are also expected to be present in other aquatic environments based on their physico-chemical properties.
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When degradation occurred, TCE was mainly transformed into innocuous non-chlorinated transformation products, while hazardous chlorinated transformation products accounted for a small percentage of the mass-balance.
Transformation products.
Ozonation transformation products.
Transformation products or metabolites were determined in 26 studies.
However, transformation products formed can still be toxic.
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