Ligation, transformation and preparation of plasmid DNA from E. coli were conducted according to standard procedures [53].
Transformation, plasmid preparation and sequencing were performed as described above.
Yeast transformation and preparation was performed essentially as described previously [ 30, 49, 50].
Then we will be dependent on a series of transformations in preparation for the convergence proof in Section 4.
To examine if the Kdm2b wt/ΔCxxC mice exhibited homeotic transformations, skeletal preparations from newborn (n = 10) and 11 dpn (n = 2) Kdm2b wt/ΔCxxC mice were compared to control Kdm2b wt/wt mice.
The small size and high copy number of the vectors are ideal for efficient cloning, transformation, and plasmid preparation.
The contribution of this work is the design and implementation of a six-phase process that includes the preparation, transformation, clustering, recognition, refinement, and revision for extracting ontology from unstructured HTML pages.
Standard protocols were followed for cultivation, transformation, mating, sporulation, preparation of genomic DNA, and tetrad dissection of yeast [68].
For cultivation transformation, mating, sporulation, preparation of genomic DNA, and tetrade dissection of yeast, standard protocols were followed [37].
