Sentence examples for transformation plate from inspiring English sources

Using this technique, up to 145 transformants were obtained per transformation plate, and a ku70 deletion strain was constructed in the ATCC 42464 background and characterized.

Up to 145 transformants per 10 conidia were obtained in one transformation plate.

By only scraping non-overlapping regions from the transformation plate, we ensured that FOAR colonies from different streaks derived from independent transformants.

In contrast, in gsSSG mutagenesis a portion of a transformation plate can be scraped using a single toothpick and struck on FOA, and this streak will almost always contain only recombinants.

A region of the transformation plate containing several hundred colonies (approximately 1/20 of plate) was scraped on to a single toothpick and then streaked to one quadrant of an FOA plate.

A starter culture was prepared by flushing colonies from the transformation plate into 500 mL of LB medium supplemented with 100 μg/ml ampicillin and then incubated at 37 °C overnight at 180 rpm in an incubation shaker.

> -wrap-foot> aNumber of primary transformants on transformation plates (TrMMsorb + uridine + chlorimuron ethyl (Als substrate)) containing 1 % of lactose and 1 % glucose referred to as induced and non-induced expression of I- SceI, respectively.

The major modifications included the automatic design of PCR primers, and improved multi-step laddered PCR, followed by previously established micro BP reaction of GATEWAY cloning, transformation, plating of transformed E. coli cells (DH5α), colony picking and entry clone plasmid DNA extraction.

Colonies were randomly picked from the transformation plates and re-plated on complete medium (Pontecorvo et al. 1953).

40 transformants were selected from the transformation plates and transferred to small malt extract plates containing hygromycin (50 μg/ml).

G402 was transformed with a LEU2 plasmid expressing either SSA1, 2, 3 or 4. Transformation plates were replica plated onto selective medium containing 5-fluoroorotic acid (5-FOA), a chemical that selects against URA3 cells and hence against the presence of the pRDW10 plasmid.