The cloning efficiency (number of clones formed per transformation) of selection-free PCRRC with pOmni was negatively correlated to the length of cloned fragments (Table 2), which is in agreement with the result in another PCRRC reported (Bryksin and Matsumura 2010).
After transformation of the selection plasmid into BL21 DE3), inhibited cell growth both on liquid and solid media was observed upon tryptophan removal for PRK expression (Fig. 3A and 3C).
The combination of EBV transformation and selection of immortalized lines that exhibit the desired antigen binding characteristics with phage display technology provides a useful strategy for specific recombinant antibody generation.
Transformation of the plasmids, selection and induction of the intron II system resulted in erythromycin-resistant colonies.
After transformation of both fragments, selection on agar medium containing 100 mg/L nourseothricin (Jena Bioscience, Jena, Germany), yielded strain IMI302.
Marker-assisted selection (MAS) involves the transformation of phenotypic to genotype selection, which can improve the efficiency and accuracy of the selection of target traits, and has been widely used for crop genetic improvement (Chen et al. 2013; Jena and Mackill 2008; Xu and Crouch 2008).
We evaluated an automatic procedure of CRC detection using MALDI-TOF spectra based on: (i) discrete wavelet transformation; (ii) selection of discriminative wavelet coefficients using the non-parametric difference tests with multiplicity adjustment; (iii) SVM classification.
The cotyledonary nodes and cotyledons from 7-day-old seedlings were used in Agrobacterium-mediated transformations with two kinds of selection strategies, shifting or consistent.
In all the cases the biolistic transformation and selection of cells were performed as already described [65], [66].
HK carried out the transformation and selection of potato plants.
In this study, we have described a simple method for transformation and selection of Ap.
