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Agrobacterium-mediated transformation of seed embryo-derived embryogenic callus was performed according to Sallaud et al. 2003.
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However, its function in grapevine remains to be proven by genetic transformation of seeded cultivars.
It differs from mere alteration, such as the browning of an autumn leaf, where an identifiable material substratum persists, and from generation and corruption, such as the transformation of an olive seed into a tree or the decay of a rose into a heap of dust, where no identifiable material substratum persists.
Increased content of monoclinic form is explained by nucleation under conditions of high CI (34% w/v), low seeding temperature (below 7 °C) and long seeding time (more than 14.7 min) in addition to transformation of grown orthorhombic seeds.
Logarithmic transformation of individual capsule means of seed dry weight, fresh weight and moisture content satisfied the requirement for normality and equal variance (with the exception of moisture content for N. pseudonarcissus).
Achieving this increase in global food production requires nothing less than a systemic transformation of the way we seed, harvest, and cultivate our food.
Christou et al. [ 13] were the pioneers in the area of biolistic transformation of soybeans using immature seed meristems.
Plant height, number of leaves, number of spikelets, and total weight of seed produced were transformed with the Box Cox transformation to meet the assumptions of homogeneity of variances and normal distribution of residuals; F1 and F2 seed size, emergence day, and number of leaves were log-transformed; other variables did not require transformation to meet assumptions of anova.
Transformations of the data improved the distribution of seed mass, but did not affect the statistical results enough to warrant transformation.
With this knowledge the authors were then able to assemble a large T-DNA construct for stable seed-specific transformation of Arabidopsis, and reported a high level of DHA (but not EPA) in seed oil [ 15 ]; a similar approach yielded lines accumulating significant ARA [ 16].
With this knowledge, the authors were then able to assemble a large T-DNA construct for stable seed-specific transformation of Arabidopsis, and reported a high level of DHA (but not EPA) in seed oil (Petrie et al. 2012a) and separately yielded lines accumulating significant ARA (Petrie et al. 2012b).
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