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"Excavations in the 1920s and 1950s uncovered large parts including the gatehouse, the moat, some interior buildings and perhaps part of the dungeon, so it's clear there's significant archaeology here". The Castlegate redevelopment will also see a new green "corridor", the transformation of empty shops and work to protect the city's Old Town Hall.
The addition of cycloheximide prevents transformation of empty plasmids, which remain circular due to incomplete ClaI and SalI restriction digest, as those vectors still maintain the CYH2 gene that confers dominant sensitivity to cycloheximide.
A double transformation of empty vectors served as negative control.
Transformation of empty vector into a ctk1Δ strain produced no change in TBP cross-linking, which was still seen in the PMA1 coding region.
Similar(56)
In controls, co-transformation of empty prey plasmid with a FOXL2, PIAS1, or UBC9 bait plasmid failed to activate expression of reporter genes.
Construction has begun on a Taco Bell restaurant at 2800 Crenshaw Blvd., marking the transformation of an empty lot that had been a glaring reminder of the 1992 riots.
She then addresses the troublesome increase in images now mediating politics and the transformation of politics into empty spectacle.
"There's so much more to Detroit than its abandoned buildings," said Jenenne Whitfield, the executive director of the Heidelberg Project, an arts organization there founded by the artist Tyree Guyton, whose sculptural transformation of a block of empty houses draws 275,000 visitors a year.
Figure 4 shows that complete deletion (69 bp) of NUMT13 (which also deletes 39 out of 229 bp of ARS1215) results in plasmid loss rate and transformation efficiencies comparable to those of empty vectors.
The adhesion contact recession and geometry transformation of HepG2 cells transfected with empty vector (pcDNA3.1 cells), wild type HBV (wtHBV cells) and mutant HBV genome (mHBV cells) are probed during the thermal transformation across lower solution critical temperature of TRP.
Transformation of S. cerevisiae EBY.WV4000 with p426HXT7-6His (empty vector), p426HXT7-6His_mstG and p426HXT7-6His_mstH was performed as described [ 57].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com