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"This was always going to be a highly ambitious and complex programme but the impact of running an IT and a contact centre transformation in parallel was underestimated: the combination of new systems, new processes and new customer services agents impacted service levels significantly".
A stretching-induced or impact-induced β-α transformation in parallel with micro-crazing was also noticed.
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Even by using yeast cells known to have worked in previous experiments, it is advisable to perform several transformations in parallel.
In future work we will use this transformation system, in parallel with transformation in heterologous plant systems, for functional testing of spruce TPS and P450 promoter constructs linked to reporter genes.
Likewise, it seems that malignant transformation appears in parallel to an upward trend of ST6Gal I activity, since transitional tissue activity values are intermediate between those of healthy and tumour ones.
The YCp- ACT1 plasmid and YCp50 were transformed in parallel into haploid deletion strains and the transformations were scored for both transformation efficiency and colony size upon restreaking.
A 1 4000 dilution of each transformation was plated in parallel to enable estimation of the number of unique transformants we obtained at least two-million unique colonies per transformation.
Vector only control transformations were done in parallel with the library transformations.
Their subsequent progressive transformation to jarosite in parallel to a pH decline was colorimetrically detected by a displacement of the spectral maximum to 450 nm, its intensity reduction with increasing jarosite content, and yellowing of aggregates and ground-soil samples from 8.5YR to 0.2Y.
These advantageous features of sonoporation-based transformation suggested that this method can be readily adapted for high-throughput or remotely controlled screening and testing of genetic transformation schemes, where a large number of transformation experiments are performed in parallel, with or without robots.
Overall, the approach delivers a contribution to theoretical and practical knowledge in the multidisciplinary research field of transformation towards sustainability and, in parallel, offers a basis or starting point for further research.
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CEO of Professional Science Editing for Scientists @ prosciediting.com