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The obtained sequences were analyzed using The Basic Local Alignment Search Tool (BLAST®) of the National Center for Biotechnology Information (NCBI) database and, BioEdit sequence alignment editor software, version 7.0 [ 30].> The positive constructed recombinant clones were used for transformation in expression prokaryotic host Escherichia coli BL21 DE3) competent cells (Novagen, USA).
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We applied a log transformation in the expression values.
This was cloned into pUAST (Brand and Perrimon, 1993) and pAc5.1 (Invitrogen) for transformation and expression in cultured cells, respectively.
Here we describe a novel system-level methodology for unbiased cloning of intact large SM clusters from a single fungal genome for one-step transformation and expression in a model host.
The significance for homeolog bias was calculated using a paired, Student's T-test with log2 transformation to ensure the normality in expression values.
There is large similarity in expression of neoplastic transformation signature genes between breast and ovarian carcinomas.
Oncogenes and tumor suppressors are a group of molecules that showed significant changes in expression during the transformation.
One explanation could be that the expression of ATPIF1 levels out after induction of metastasis, and only increases in expression during the transformation process from normal cell to tumour cell.
In tomato transformation studies, expression of CsLCYb1 under the control of the cauliflower mosaic virus (CaMV) 35S constitutive promoter resulted in a virtually complete conversion of lycopene into β-carotene, and the ripe fruits displayed a bright orange colour.
Repetitive elements are important features of pathogenic Neisseria genomes, playing key roles in genetic transformation, gene expression and genome rearrangements [9], [10].
RR carried out Pichia pastoris transformation and expression, participated in the immunofluorescent labeling.
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