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No differences in mitotic stability, copy number and transformation frequency were observed.
Transformation and analysis of the relative transformation frequency were performed as in reference [27].
Thirdly, the effects of sonication power and time on transformation frequency were investigated.
Secondly, the effects of co-cultivation duration and acetosyringone concentration on transformation frequency were optimized separately.
If transformation frequency were uniform across the genome, the donor allele frequency would be expected to decrease exponentially with increasing distance from the selected site (Croucher et al. 2012), but this was not seen.
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Transformation efficiency was computed as transformants per microgram of DNA, and transformation frequency was expressed as transformants per microgram of DNA per viable cell.
Transformation frequency was expressed as percentage of transformants against total viable recipient cells per milliliter cell suspension.
The transformation frequency was calculated as the ratio of transformants that exhibited both resistance against paromomycin and the correct aphVIII fragment following PCR and the total number of cells used for each transformation experiment (9 × 10 cells).
Transformation frequency was calculated as the ratio of the transformant number to the recipient (competent) cell number.
Transformation frequency was calculated as the ratio of the transformant number to the estimated recipient cell number, which was regarded as half of the total cell number in each sample.
Unexpectedly, increasing the amount of this mixture did not increase the number of transformants per plate in a dose-dependent manner because the transformation frequency was reduced (Table 4).
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