Sentence examples for transformation frequency to from inspiring English sources

Increasing the ratio of A. tumefaciens to target conidia to 1 × 104 improved the transformation frequency to 30 transformants per 1 × 104 conidia (Table 3, 48 h) on average; the single-copy integration of the T-DNA decreased to 30%, but the frequency of T-DNA integration into a single locus with the vector backbone typically increased to 55%.

The transformation frequency declined with an extended pre-culture time, with a 6-day pre-culture resulting in a decline of the transformation frequency to 51.11%.

Designated Ensifer adhaerens OV14 (OV14), this strain can deliver sufficient transformation frequencies to present Ensifer-mediated transformation (EMT) as a viable alternative to existing transformation technology platforms [ 12].

Interestingly, in SMM the non-induced pLK containing strains showed a 2 to 6 fold increase in transformation frequency compared to the control strains carrying empty plasmid (Table 2).

Assuming that all of the E. elegans colonies used for transformation contained 32 cells, the calculated transformation frequency corresponds to 1.2 × 10-5 (± 14%) transformants per organism.

The transformation frequency tends to vary among different isolates of the same fungal species when AMT is performed using a method optimized for the standard strain (Roberts et al. 2003; Sullivan et al. 2002).

DU-uranyl chloride treatment resulted in a 9.6 (+/- 2.8 -fold increase in transformation frequency compared to untreated cells.

Therefore, for stacking of more than two genes in cisgenic transformation, the effect of an increased insert size (e.g. >11 kb) on transformation frequency remains to be tested.

Ethidium bromide-resistant transformants were selected in a single step, but at a transformation frequency of 10−6 to 10−7, 10- to 20-fold lower than that for transformants transformed with a spectinomycin resistance cassette under the same conditions.

Observed cotransformation frequencies of NovR and NalR were ~10-fold higher than predicted by the product of their single marker transformation frequencies (6.2- to 14.5-fold, Table 1), and these values were consistent for two donor DNAs, both the RR3131 donor DNA and DNA from an Rd-derived strain MAP7 (RR666) (t-test p-value = 0.26).

Further, CEC2 with swapped positions of xynA and aadA displayed ~two orders of magnitude lower transformation frequencies compared to other constructs (data not shown), which implies inadequate aadA expression (probably at the transcriptional level), necessary to support selective regeneration of transplastomic clones.