Sentence examples for transformation frequency of from inspiring English sources
We consequently obtained a maximum transformation frequency of 30 transformants per 1 × 104 conidia, on average, when 1 × 104 conidia of A. aculeatus were mixed with 1 × 108 bacterial cells (OD660 = 0.8) and co-cultivated for 48 hours on IM plates.
The deletion of comI had a beneficial effect on the transformability, as CM1 displayed a doubled transformation frequency of 201% ± 4.6, an effect that is nevertheless 50-fold lower than the effect observed in B. subtilis NCIB3610, in which the deletion of comI resulted in an approximately 100-fold increase of the strain's transformability (Konkol et al. 2013).
Ethidium bromide-resistant transformants were selected in a single step, but at a transformation frequency of 10−6 to 10−7, 10- to 20-fold lower than that for transformants transformed with a spectinomycin resistance cassette under the same conditions.
In the εs = 125, an increase in the transformation frequency of γ → ε leads to a large density of intersecting zones of ε-phase crystals.
All 3 nickel compounds significantly (p < 0.05) increased the transformation frequency of RTE cells to EGVs at moderately cytotoxic concentrations; the order of potency was Ni3S2 > NiO = NiSO4.
Transformation frequency of the conjugation is expressed as the number of transconjugants per output donor.
Tracing changes in transformation frequency on each of the four trees indicated that all strains with a change in uptake had a change in transformation.
Transgenic plants were produced with a 21.1% transformation frequency on the basis of bialophos selection.
When acetamide utilization, uridine prototrophy, phleomycin resistance, or hygromycin resistance was used to select transformants in A. awamori, average transformation frequencies of 0.2, 40, 80, and 200 transformants per 106 conidiospores were obtained (Michielse et al. 2008).
A large library also requires good electrocompetent E. coli cells with transformation frequencies of 10-10 transformants per μg plasmid DNA.
Figure 1 shows the log-transformed means (±SEM) of the transformation frequencies of serotypes 6B, 14, 19F, 9V, 23F, 3, and 18C induced by CSP-1 and CSP-2.
