Sentence examples for transformation frequency in from inspiring English sources
The transformation frequency in C. reinhardtii was calculated as 1.3 - 1.9 × 10-7 transformants per cell [ 87], which is similar to the values above.
The transformation frequency in B. licheniformis MW3.1 was arbitrarily set as 100 (Fig. 2c).
Although the transformation frequency in liquid IM was 100-fold lower than that on solid IM, the AMT method using liquid IM is better suited for high-throughput insertional mutagenesis because the transformants can be isolated on fewer selection media plates by concentrating the transformed germlings.
Although the transformation frequency in liquid IM showed a 100-fold reduction compared with the solid IM, this transformation method is suitable for random insertional mutagenesis because fewer SM plates are required for the transfer of transformants from IM to SM plates.
Therefore, we attempted to elucidate the mechanism of high transformation frequency in CAG18439 as follows (Table 7 and Figs. 3 6).
These results suggest that unidentified mutation(s) in the CAG18439 chromosome other than Tn10 and lacI cause the high transformation frequency in CAG18439.
Transformation frequencies in marker-free transformations (MF) are derived from the number of transformed shoots (transformed) and the total number of shoots minus the number of transformed shoots (non-transformed).
However, treatment of cells with penicillin (10 µg/ml) increased transformation frequencies in both phases, with major improvement (100-fold) for late-log phase cells.
There is limited information about transformation frequencies in other algae with the exception of some diatom species.
On an overall basis, the transformation frequencies in volvocine algae vary by about two orders of magnitude (1.1 × 10-7 to 2.5 × 10-5).
Transformation frequencies in marker-assisted transformations (MA) are derived from the number of rooting shoots (transformed) and the number of explants used minus the number of rooting shoots (non-transformed).
