Sentence examples for transformation frequencies with from inspiring English sources
In spite of the increasing range of transformation frequencies with increasing genomic divergence, the DNA positions that yielded the highest transformation frequencies with GMI1000 (TCP3, TCP6, and TCP7) systematically yielded the highest frequencies for each recipient (Figure 5).
Therefore, the reduced differences found between the lowest and the highest transformation frequencies with genomic borne fragments could be explained by the involvement of Chi-like sequences at some unknown distance upstream.
In contrast to the single copy of comE1 in the Pasteurellaceae, there are four copies of comE in the N. gonorrhoeae genome and serial deletions resulted in decreases in transformation frequencies, with a reduction of 4×104-fold 4×104-foldwhen all copies wereductiond [6].
Transformation frequencies with NHEJ-deficient strains are often low, making it difficult and laborious to generate enough mutants for library screening.
The transformation frequency with ptrA can reach 30 50 transformants per μg of DNA.
Decrease in transformation frequency with increase in bacterial intensity was observed.
CCM pH below or above 5.4 resulted in the decrease of transformation frequency, with the lowest being 10.31% at a pH of 6.0.
The lower transformation frequency with DNA from a diverged background is presumably due to sequence heterology around the selected alleles, which could either reduce the probability of strand exchange or increase the probability of recipient-directed heteroduplex correction.
A large library also requires good electrocompetent E. coli cells with transformation frequencies of 10-10 transformants per μg plasmid DNA.
Moreover, the range between the highest and the lowest transformation frequencies also increased with overall genome divergence reaching more than 4 orders of magnitude for strains NCPPB332 and CFBP2957 while it was less than two orders of magnitude for the two most closely related strains (GMI1000 and CFBP2968).
The transformation frequency obtained with stem sections with one node as explants was significantly different (Fisher's Least Significant Difference (LSD) test; P < 0.05) to all other types of explants tested.
