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Transformants from five independent transformation experiments (i.e., 33 clones) were tested for their maximal antibiotic resistance.
Transformation experiments will make use of the leuphenotype and selection of transformants using LEU2 complementation.
Based on 5 independent transformation experiments, the transformation frequency was calculated as 3.7 × 10-7 (± 14%) transformants per cell.
f-j Binary vectors for Agrobacterium-mediated transformation experiments.
Rice (Oryza sativa L. ssp. japonica) cultivar Nipponbare was used for protoplast isolation and transformation experiments.
Special silica ampoules were designed for the anaerobic and hydrothermal in-situ transformation experiments.
Curing and transformation experiments showed that resistance to amikacin was plasmid-mediated.
Escherichia coli DH10B and Agrobacterium tumefaciens strain EHA105 were used for cloning and transformation experiments.
Therefore, only data produced from the DH5α strain are reported for these and subsequent transformation experiments.
Subsequently this plasmid-containing strain can be used in multiple transformation experiments.
Transformation experiments with pPBg12EGFP yielded 7 different founder families, representing a minimum transformation efficiency of ∼13% (Table S2).
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