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A japonica rice variety, 9804, was used as the recipient to transform the maize Rxo1 gene in our transformation experiment using the standard Agrobacterium mediated transformation system, as described previously [ 40].
In Gonium, the estimated efficiency of transformation was based on two assumptions, which were that all the cells that were used in a transformation experiment were bombarded by microprojectiles and that only a maximum of one transformant arose per cultivation flask.
Air mass transformation experiment.
Transformation experiment was set up in a randomized design with three replicates of 15 explants each.
We show that it is possible to introduce two point mutations and one gene insertion in one transformation experiment with a very high efficiency.
By this approach, a microbial community, typical for authentic storage tanks, is used in the transformation experiment, which is not the case if excrements are sampled from diverse animals and mixed afterwards in order to obtain a manure-like medium.
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Transformants from five independent transformation experiments (i.e., 33 clones) were tested for their maximal antibiotic resistance.
Transformation experiments will make use of the leuphenotype and selection of transformants using LEU2 complementation.
Based on 5 independent transformation experiments, the transformation frequency was calculated as 3.7 × 10-7 (± 14%) transformants per cell.
f-j Binary vectors for Agrobacterium-mediated transformation experiments.
Rice (Oryza sativa L. ssp. japonica) cultivar Nipponbare was used for protoplast isolation and transformation experiments.
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