Transformation of Escherichia coli One Shot® MAX Efficiency® DH10B™ cells (Invitrogen, Carlsbad, CA) was conducted by electroporation (transformation efficiency of 107 transformants/µg DNA) followed by immediate plating onto ampicillin-supplemented LB agar plates.
In conclusion, with the optimized approach, a high transformation efficiency of 145 transformants per 10 conidia was obtained.
The second round was performed with Top 10 cells that have a transformation efficiency of 10 transformants/μg of plasmid DNA.
The use of overexpression of pporRFP and tracking analysis of transformed tissue has enabled important increases in transformation efficiency of switchgrass [ 29].
We have proposed a simple but promising strategy for improving the transformation efficiency of L. lactis and other hard-transformed microorganisms.
Due to the low transformation efficiency of mycobacteria, the two plasmids were transformed into M. smegmatis sequentially and selected on hygromycin and kanamycin.
Hygromycin-resistant clones transformed by the vectors appeared with a common transformation efficiency of approximately 1,500 per 1.6 × 106 electroporated cells.
Puromycin-resistant clones transformed by the vectors appeared after transfection with a transformation efficiency of approximately 1,300 per 1.6 × 106 electroporated cells, irrespective of the vector differences.
The transformation efficiency of D. rotundifolia L. was 17%.
There is, however, an outstanding difference regarding the transformation efficiency of the two linearized plasmids.
A total of 23 PCR positive events were obtained with a transformation efficiency of 5% around.
