The majority (~90%) of suspension culture lines derived from calli that were obtained directly from transformation consisted of cells with various levels of GFP fluorescence.
Analysis of GFP fluorescence in primary suspensions obtained either directly from transformation or from secondary and tertiary clones revealed the coexistence of cell populations with different T-DNA insertions in some primary lines, representing genetic heterogeneity.
By analysing GFP fluorescence in tobacco BY-2 cells, we found that expression of GFP transgene was highly heterogeneous in the majority of transgenic lines obtained directly from transformation.
The practical consequence of this much higher efficiency of marker replacement is that in gsSSG mutagenesis recombinants can be isolated by streaking colonies directly from transformation plates to FOA medium (see Methods).
It was then less than a month after the terrorist attacks of Sept. 11, and the show's ritualistic portrayal of love, death and transformation somehow seemed to flow directly from the collective unconscious of a stunned city.
Despite the mythology, the nation only became leftwing in its electoral choices during recent decades – and this transformation in large part derives directly from the experience of the 1980s.
When Mary Zimmerman's dreamlike adaptation of Ovid's tales opened in the fall, it was less than a month after the Sept. 11 terrorist attacks, and the show's ritualistic portrayal of love, death and transformation somehow seemed to flow directly from the collective unconscious of a stunned city.
*+ "METAMORPHOSES" (Tony winner for best direction of a play.) When Mary Zimmerman's dreamlike adaptation of Ovid's tales opened in the fall, it was less than a month after the Sept. 11 terrorist attacks, and the show's ritualistic portrayal of love, death and transformation somehow seemed to flow directly from the collective unconscious of a stunned city.
(Tony winner for best direction of a play.) When Mary Zimmerman's dreamlike adaptation of Ovid's tales transferred to Broadway last fall, it was less than a month after the Sept. 11 terrorist attacks, and the show's ritualistic portrayal of love, death and transformation somehow seemed to flow directly from the collective unconscious of a stunned city.
When Mary Zimmerman's dreamlike adaptation of Ovid's tales opened in the fall, it was less than a month after the terrorist attacks of Sept. 11, and the show's ritualistic portrayal of love, death and transformation somehow seemed to flow directly from the collective unconscious of a stunned city.
