Sentence examples for transformation data not from inspiring English sources
Without such thermostable genetic marker systems, false positive colonies would grow on plates after transformation (data not shown).
In our hands, the naïve, unsupervised equal-width interval binning discretization proved superior to other schemes of data transformation (data not shown).
There was one outlier for HbA1c (changed from 10.5% at baseline to 7.9% at 26 weeks), and the results were similar using a rank transformation (data not shown).
No colonies were seen on selective plates in controls lacking recipient L-forms, donor plasmid, or PEG treatment for transformation (data not shown).
Nevertheless, VvMYB5bL was still functional despite a growth delay on solid selective medium (6 days) compared to VvMYB5bR recombinant yeasts that were able to develop 4 days after transformation (data not shown).
Our data show that ectopic expression of PDK1 induces MYC signaling and cellular transformation (data not published); conversely, pharmacologic or genetic removal of PDK1 reduces MYC signaling and alleviates rapamycin resistance.
Following successful gfp insertion (Fig. 3C) direct induction of the resulting clone copy-number provided, from a single overnight miniculture, quantities of high-quality DNA sufficient for numerous C. elegans transformations (data not shown and Fig. 2D).
Control transformations of linearized plasmid DNA alone or PCR amplicons alone did not yield a significant number of transformants (< 20) while the 'complete' transformation contained several hundred transformants (data not shown).
Further studies demonstrated that by reducing the concentration of DNA used for transformation, the number of such double transformants could be reduced and also result in higher overall transformation efficiency (data not shown).
Two days co-cultivation period was found best for H. isora transformation (detailed data not shown).
To test our hypothesis, we performed a Southern blot analysis with genomic DNAs isolated from antibiotic-resistant calli and found that these hygromycin-resistant calli carried the hygromycin-B-phosphotransferase gene, the antibiotic marker gene of the pOsSRT1::OsSRT1-RNAi plandid and originated from different transformation events (data not shown).
