Exact(1)
As the lines S1-COXS3-22.3 and S1-COXS3-22.15 were derived from the original TR1-phiC31.22 secondary transformation; crossing them back to the TA3-phiC31.22 target line was performed as a positive control for this line of investigation.
Similar(59)
We introduced the targeting construct into w1118 flies by germline transformation, crossed transgenic females containing the donor construct inserted on the X chromosome with males carrying transgene insertions on the 3rd chromosome that express the FLP and I-SceI under control of the hsp70 promoter (stock 4).
Time-specific effects are removed by subsequently applying the within transformation cross-sectionally, i.e., begin{aligned} tilde{Y}_{i,t}:=ddot{Y}_{i,t}-N^{-1}sum _{i=1}^N ddot{Y}_{i,t}, end{aligned} (4 where N is the total number of stocks in the sample.
I tell them that while individual organisms are not capable of "evolving" or "adapting themselves," there are changes in the genetic makeup of populations over time as a result of mutation, transformation, transduction, crossing over between homologous chromosomes, polyploidy, loss of alleles from small populations, and other processes.
Studies on B. distachyon benefit from rapidly developing community resources, such as a completely sequenced genome, mutant collections (both chemically derived and sequence-indexed DNA insertions), and efficient protocols for transformation and crossing [ 2- 5].
Both model transformation and cross terms bounding techniques are avoided in the derivations.
To determine whether these mutant phenotypes were caused by the ura4+ insertion or mutations induced by transformation, we crossed the mutants to a wild type strain and performed tetrad analysis on a subset of the identified mutants to verify co-segregation of the ura4+ marker and the phenotypes.
More importantly, all of the model transformations, cross-terms bounding techniques and free additional matrix variables are avoided in the derivation, so the results obtained have less conservatism and simpler formulations than the existing ones.
In a coming-of-age transformation, Anthony is crossing to the positive side.
Rituals are about transformation, the crossing of a threshold, and in the case of the bedside exam, the transformation is the cementing of the doctor-patient relationship, a way of saying: "I will see you through this illness.
Yeast strains were generated by standard transformation and crossing protocols.
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