Sentence examples for transformation conditions from inspiring English sources

Unfortunately, numerous attempts [including a variety of different strains, markers (hph-NT1 and URA3MX4), and transformation conditions] failed to yield transformants with the desired deletion.

Although it has been previously demonstrated that A. tumefaciens is capable of transforming various fungi including the Ascomycetes, the transformation conditions must be optimized because the transformation frequencies vary among fungal species and strains.

It is easy to show that under the above transformation conditions (H2 - H4) still hold.

Then, the relationships and transformation conditions of these models are investigated.

The transformation frequency is also affected by slight differences in transformation conditions or the physiological state of the recipient cells (Michielse et al. 2005a).

In this study, regeneration and transformation conditions, including explant selection and surface sterilization, use of different Agrobacterium strains, and co-culture processing, are optimized.

Betulin biotransformation was carried out with the filamentous fungi Armillaria luteo-virens Sacc QH (ALVS), Aspergillus foetidus ZU-G1 (AF) and Aspergillus oryzae (AO) under seven kinds of transformation condition.

If the wall thickness is large enough, the tangential stress may vanish at the inner radius and correspondingly the stress state may reach a corner of the Tresca transformation condition, thus originating two different Martensite variants according to the corner transformation rule.

The influential factors were investigated systematically and the optimal transformation condition was achieved using hypocotyls as explants, including the sonication treatment of 10 min with 80 W, A. tumefaciens infection of 30 min and co-cultivation of 2 d in 1/2 MS medium containing 100 μM acetosyringone.

However, the varying co-transformation rates reported for different volvocine species may not represent species-specific values, but they could be the result of different plasmid sequences, plasmid sizes, or (co- transformation co- transformation

The latter response is intriguing in the light of the previous observation that Wnt-5A failed to induce transformation under conditions where Wnt-1 was highly transforming [ 8].