Sentence examples for transformation as previously from inspiring English sources

The three constructs were introduced into rice using Agrobacterium-mediated transformation as previously described (Kim et al., 2009) with minor modifications.

Nipponbare calli transformation of this construct has been done through Agrobacterium tumefaciens-mediated stable transformation as previously described (Sallaud et al. 2004).

To study the expression pattern of SFAR4, Pro SFAR4 :GUS transgenic plants with the β-glucuronidase (GUS) reporter gene under the control of the SFAR4 promoter were cloned into the pBI101 plasmid, then generated by Agrobacterium tumefaciens-mediated genetic transformation as previously described.

The strain cuf1Δ was generated by knocking out the gene CDS_2478 using biolistic transformation as previously described [6].

To characterize the function of Hrk1, the HRK1 gene was deleted in the genetic background of C. neoformans serotype A strain H99 (MATα) and KN99a (MATa) with a disruption cassette generated by overlap PCR and by biolistic transformation as previously described [5], [27].

The purified cassettes were used for yeast transformation as previously described.

Cells were grown to mid-exponential phase (OD730nm of 0.4 1) and natural transformation performed as previously described with some modifications [ 23].

The resultant transformed plasmids were used for Agrobacterium-mediated rice transformation as described previously [31].

The resulting construct was linearized by EcoRI before being transformed into Gransden 2004 wild-type protoplasts [ 52] using PEG mediated transformation as described previously [ 53].

via Agrobacterium-mediated transformation, as described previously (Wakasa et al., [2007]).

The addition of Al2O3 to ZrO2 also delays this phase transformation as reported previously [8, 11].