Sentence examples for transformation and expression from inspiring English sources
For overexpression of Rub1, pRD54-HA-Rub1 or pRD54-HARub1ΔGG was transformed into S. cerevisiae S288c using one-step transformation, and expression was induced by addition of 2% final concentration galactose.
The chloroplastic localization was confirmed by stable transformation and expression of the Am-MDAR-GFP fusion protein in tobacco.
Particle bombardment was employed for transformation, and expression of Shble under the control of TUB and UEP promoters, cloned from N. salina, was used to confer resistance to Zeocin antibiotics, resulting in 5.9 and 4.7 transformants per 108 cells, respectively.
While Fosso used these images to communicate his well-being to his grandmother in Nigeria, the staged self-portraits also became agents of transformation and expression for the young artist, who was acutely aware of shifting cultural and political climates.
For transformation and expression studies, E. coli DH5α and E. coli BL21 (DE3) were cultured in Luria Bertani broth (LB) and incubated over-night at 37 °C.
Activation of the cells was also manifested by lymphocyte blast transformation, and expression of the activation marker, CD25.
To generate a PCF derivative of EATRO795 in which RNAi could be performed, the individual pLew29 and pLew13 plasmids were introduced by transformation, and controllable expression was tested by monitoring expression of a reporter gene subsequently introduced under the control of the T7 RNA pol promoter (data not shown).
The main part of the variability (11.7%) arises from the specific lipase enzyme assay whereas the coefficient of variance concerning transformation, growth and expression steps represents only 7.2%.
Yeast transformation efficiency and expression cassette insertion were increased by constructing a strain containing a zeta docking platform for targeted integration into the genome.
Here, 1) we provide a historical perspective on advances in H. jecorina molecular biology, 2) outline host strain engineering, transformation, selection, and expression strategies, 3) detail potential pitfalls when working with this organism, and 4) provide consolidated examples of successful cellulase expression outcomes from our laboratory.
The construction of recombinant plasmids, transformation and protein expression were performed according to the protocol described by Sambrook and Russell [ 36].
