Tetracycline resistance determinants were investigated by plasmid profiling, transformations, and amplification of plasmid-borne resistance genes.
Following vector construction, transformation and zeocin amplification, the best Pichia producers were identified in a screening procedure using Western blot and a Luminex xMAP™ based high-throughput method.
We further investigated the synergistic tumor-suppressive effect of CnB and IFN- γ in vitro and in vivo and explored possible mechanisms of both the macrophage transformation and the interferon signaling amplification.
After hybridization, ligation, and transformation, we used amplification of u-series universal primers and subsequent sequencing to identify the positive clones.
Indeed, MR draws upon complex mathematical concepts, requiring its users to be familiar not only with computer programming but also with radio-wave detection and amplification, electrical filtering, Fourier transformation, and complex time-domain modelling and integration.
The discrete SDF-1 product was subsequently TA cloned into pcDNA4/GFP-NT vector (Invitrogen Ltd), followed by transformation using One-Shot E. coli (Invitrogen Ltd), verification, and amplification.
The PCR amplifications, biolistic transformation and confirmation of acapsular morphology was according to standard methods [10], [15], [16].
Its overexpression and amplification are both known to be associated with neoplastic transformation in CD30-positive lymphomas through an NF-κB-dependent mechanism.
