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In order to monitor transformation activity of A. tumefaciens, GV was transformed with the binary vector pCP60-35S-DsRed2 to yield GV3101 pMP90)/pCP60-35S-DsRed2 (abbreviated – GVR).
In prior work, we established human embryonic kidney epithelial cells engineered to express hTERT, the SV40 Early Region and an activated allele of MEK as a model system for demonstrating transformation activity of oncogenes, including AKT1 and IKBKE [28].
The fdr gene encoded a novel ferredoxin reductase whose absence resulted in lower transformation activity of carbazole by CarAa and CarAc.
Furthermore, when the senescence programme is completely abrogated by E1A oncoprotein, ectopic HMGA2 promotes transformation activity of ras both in vitro and in vivo (Narita et al, 2006).
We used the soft agar assay to evaluate the cell transformation activity of arsenite exposure and the nude mice xenograft model to determine the tumorigenesis of arsenite-induced transformed cells.
Given previous reports that the transformation activity of KRAS is dependent on RAC1 (Qiu et al., 1995), it seems likely that RAC1 would remain an efficacious target even in the setting of a KRAS mutation.
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In concert with the morphological cell transformation activities of other K-region dihydrodiols of PAHs, these data suggest a new mechanism/pathway for the morphological cell transforming activities of B[a]P and its metabolites.
The MCF10A series of cell lines has frequently been used to study transformation activities of oncogenes and tumor suppressor genes.
Thus, it is possible that overexpression of WRAP53 facilitates the cell transformation activities of telomerase or vice versa.
Transformation activities of insects and insect symbionts toward trichothecenes have been studied (Dowd 1992; Dowd and van Middlesworth 1989), but de-epoxidation has not been observed, indicating that insects may use other mechanisms to protect themselves against trichothecenes.
Through a correspondence factor analysis, this qualitative material could be useful to build a typology of the "transformation activities", to which are associated specific coordination mechanisms and "circulation activities".
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