Sentence examples for transformants would be from inspiring English sources

When four codons are mutated, a pool of ~10 E. coli transformants would be required to ensure detection of the rarest combinations.

The simplest mechanism to generate white Ade+ Ura+ transformants would be by invasion of Chr XV containing the ade2 -I allele by the CFV and copying the 3′ part of the ade2 gene followed by displacement of the invading strand and invasion of the ade2 -n allele copying the 5′ part of the gene, resulting in formation of a functional ADE2 gene carried on the CF, though other mechanisms are possible.

We first concentrated on recurrent mutations transferred to both transformants as we hypothesized that these would be the most likely to be responsible for resistance.

It was expected that roughly equal numbers of transformants with the transposon inserted in each copy of patA would be obtained.

Since our constructed expression vector used the promoter and terminator regions of the H. fabianii J640 glucoamylase gene, we expected that foreign gene expression in the transformant would also be induced by maltose.

Anticipating that the majority of the transformants would carry extrachromosomal ura4+ circles, the initial transformants were re-grown twice on non-selective medium to allow cells to lose unstable ura4+ circles.

We argued that – compared to a random mutagenesis strategy [ 15] – targeting insertion mutations towards expressed genes would increase the proportion of transformants displaying altered properties, and would decrease the total number of transformants to be screened to find a particular change in phenotype.

Frozen stocks of single transformants and pools of transformants were prepared by standard methods.

Note, we carefully chose transformants expressing the same level of Mad3p, so any stabilising effect of the ken30 mutation would not be apparent in our overexpression analysis.

These transformants were named transformant cphA6803# 1and transformant cphA6803 2.

The plasmid from one transformant remains to be identified.