Sentence examples for transformants with single from inspiring English sources

Transformants with single copy insertions were determined by segregation analysis of the T1 generation and homozygous T2 lines were identified by screening seedlings for hygromycin resistance and GUS expression.

Replicon typing was conducted on transconjugant or transformant with a single plasmid encoding blaNDM.

Ethidium bromide-resistant transformants were selected in a single step, but at a transformation frequency of 10−6 to 10−7, 10- to 20-fold lower than that for transformants transformed with a spectinomycin resistance cassette under the same conditions.

Subsequent to transformation process and selecting transformants (as single colonies on LB broth containing ampicillin, as an antibiotic resistance marker), the plasmid was extracted from transformants E. coli and treated with double digesting using BamHI and NdeI to confirm the successful transformation.

After 24 h culture and induction, transformants with the smallest halos that means single pel168 gene integration in the genome were cultured in 50 ml BMGY liquid medium for 48 h.

After the multicopy transformants appeared, single clone was cultured in 5 mL BMGY medium at 28°C with shaking at 250 rpm for 24 h.

As control, a sample of transformants with an altered morphology was purified through single conidial isolation.

3G86.1 and 3G86.32 come from the same initial clone that turned out to be a double transformant; single transformants were obtained by plasmid extraction and retransformation.

Each transformant had a single band with different sizes, indicating that the transformants contained a single copy of Sh ble integrated at different locations of the genome.

From the transformants, those with a single copy of T-DNA insertion (based on the 3 1 segregation of antibiotic resistance in T2 progeny) were isolated and homozygote transgenic plants were further identified in the T3 generation based on the segregation in antibiotic resistance.

(B) Pigmented colonies formed in transformants of alb1 with pAUR-pksP (left), but transformants with pAUR325 remained albino (right).