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Transformants with only disruption of Cbs and with both introduction of Sam 2 and disruption of Cbs were named ZJGSU2 and ZJGSU3, respectively.
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Only transformants with full-length FHL2 grew on selective plates indicating protein protein interaction.
Only transformants with CALM mutants containing amino acids 294 335 grew on selective plates indicating protein protein interaction.
Finally, a library size of 2.6 × 10 transformants was obtained, while the control reaction with only linearized plasmid gave 4 × 10 transformants.
To prevent cross contamination, each plate was inoculated with only one transformant.
Similarly, Ade+ Ura+ or Leu+ Ura+ transformants were only rarely recovered from transformation with the replicating plasmid pRS416.
The MHT identified carbapenemase activity in all transformant strains, although 3 were positive with only 1 of 2 carbapenems (Table 3).
Plants with only two components, such as PC transformants and PBcpl1 transformants, did not produce anthocyanins more than control plants (Table 1 and data not shown).
With only few exceptions, all the picked transformants produced active HRP; however, on average only three of five showed comparable growth and productivity.
Many transformation methods, such as ballistic transformation [ 7] or whiskers transformation [ 8] yield a high proportion of transformants with highly differing numbers of transgenes and only a small proportion of primary transformants with low copy numbers.
As shown in Fig. 3, a significant increase in glucose oxidase activity was observed after oxidant treatment and heat shock only in the transformants with pGX-B1, not with pGX-B0.
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