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The fact that 35S2∶MIR168b transformants with lower than wildtype AGO1 levels exhibit a lower 21/22 ratio than wildtype plants whereas the mir168a-2 mutant with higher than wildtype AGO1 levels exhibits a higher 21/22 ratio than wildtype plants suggests that preferential stabilization of miR168 by AGO1 only affects the 21-nt miR168 species but not the 22-nt species.
In tomato we observed that complementation of SlMLO1 loss-of-function mutants with CsaMLO8 restored PM susceptibility, with individual transformants with higher CsaMLO8 expression generally being more susceptible to PM than transformants with lower CsaMLO8 expression (Fig. 2).
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Ethidium bromide-resistant transformants were selected in a single step, but at a transformation frequency of 10−6 to 10−7, 10- to 20-fold lower than that for transformants transformed with a spectinomycin resistance cassette under the same conditions.
With Lower Dens.
Ballet combined with "lower" forms?
The seed yields of these transformants were lower than those of the wild-type plants.
Solidarity with lower leagues.
In general, the targeted transformants show lower background expression, a higher induction ratio, and lower variation among cells than the illegitimate transformants.
Transformation of the alb1 mutant with pAUR-PksP yielded transformants with black conidia, whereas all transformants of alb1 with pAUR325 remained albino (Figure 3B).
(B) Pigmented colonies formed in transformants of alb1 with pAUR-pksP (left), but transformants with pAUR325 remained albino (right).
Transformants with elevated laccase production might be generated by modifying the pGL promoter region and/or by screening among a larger number of co-transformants.
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