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Transformants with low levels of PKA activity are blocked in the activation of gene expression regulated by blue light.
Many transformation methods, such as ballistic transformation [ 7] or whiskers transformation [ 8] yield a high proportion of transformants with highly differing numbers of transgenes and only a small proportion of primary transformants with low copy numbers.
In these cases it is important to identify promising candidates for further breeding at an early stage, and hence being able to distinguish transformants with low copy numbers (one or two) from those with high copy numbers is sufficient.
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The transformants with the lowest resistance to paromomycin treatment were capable of surviving in 33-fold higher concentrations of paromomycin than parental wild-type strains, which tolerated only up to 0.15 μg paromomycin/ml.
The standard deviation in experiments with low numbers of transformants is substantial but not unexpected [ 28].
The transformants had low CMO enzyme activity, and produced little GlyBet (⩽70 nmol g−1 fresh wt).
With Low Cut Connie.
CRASP-3 and CRASP-5 expressing transformants bound low amounts of CFH (Fig. 4A).
For OnExpansin, GUS activity in the leaves of all promoter transformants was low.
To allow for selection of transformants with even weak transgene-mediated resistance, the lowest concentration of the antibiotic paromomycin that kills all wild-type Pandorina cells was determined.
To allow for selection of transformants with even weak transgene-mediated resistance, the lowest concentration of the antibiotic paromomycin that kills all wild-type Volvox cells was determined.
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