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Our diurnal measurements are consistent with these expectations, as transformants with increased SBPase activity showed the greatest increases in carbon assimilation relative to wild type plants around midday when light levels were highest.
Following a preliminary screening of the transformants with increased UOX activity in permeabilized yeast cells the optimal cultivation conditions for maximal UOX yield namely a 40-fold increase in UOX activity were determined.
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Stable transformants bearing UOX under control of the AOX promoter with increased UOX enzyme activity were screened by a plate patches assay in solid glucose-containing medium.
The expression of Chi67-1 in transformants was increased significantly compared with the wild type strain (P < 0.05).
The number of transformants increased with time when E. coli pEGFP cells grew exponentially in rich medium, but not in stationary phase or when inoculated in freshwater.
Representative results of this analysis, exemplified for one transformant (mt-eGFP1), are shown in Fig. 2. As expected, fluorescence of a middle-aged Mt_eGfp transformant was found to increase with increasing amounts of copper in the growth medium (Fig. 2A).
The strength of potential interactions was assessed by spotting transformants on SD/-His/-Leu/-Trp supplemented with increasing amounts 3-aminotriazole.
The transformants exhibited increased Fe-deficiency tolerance and grew well in alkaline soil.
Transformants or wild type strains were transferred into glass tubes with increasing concentrations of paromomycin in JM.
In extracts from cells treated simultaneously with E2 and RA, the number of transformants increased by an amount similar to that after treatment with E2 alone with extract from ER-positive cells.
In a gene-expression study aimed at overproduction of bacterial levansucrase using cotransformation of low-protease A. niger mutant AB1.13 (Mattern et al. 1992; Punt et al. 2008) with the A. niger pyrG gene, a nonacidifying A. niger transformant showing increased growth on medium with inulin as a sole carbon source was isolated (E. Wanker and P. Punt, unpublished results).
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