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Transformants with elevated laccase production might be generated by modifying the pGL promoter region and/or by screening among a larger number of co-transformants.
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Introduction of the BCAL1755/BCAL1756 region into B. vietnamiensis G4 (see Additional file 1 Table S2 for construct information) which lacks homologous genes in its genome did not result in transformants with an elevated resistance to amikacin.
Therefore we first investigated growth of the transformants on medium supplemented with elevated levels of glucose (2% instead of 1%).
Their E. coli transformants gained a β-lactam resistance pattern with elevated MICs of carbapenems.
Peri-implantitis increases with elevated HbA1c.
On average, electron transport rate (ETR) was significantly higher for transformants at elevated [CO2] (simple effect test; F1,12 = 8.43 p < 0.05).
Pepper your route with elevated climbs.
Transformation of the alb1 mutant with pAUR-PksP yielded transformants with black conidia, whereas all transformants of alb1 with pAUR325 remained albino (Figure 3B).
(B) Pigmented colonies formed in transformants of alb1 with pAUR-pksP (left), but transformants with pAUR325 remained albino (right).
Co-cultivation for 60 hours yielded more transformants than at 48 hours; however, more transformants with growth defects tended to emerge on the SM plates.
The size of the nanobody library is 8 × 108 transformants with great diversity.
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