Sentence examples for transformants while in from inspiring English sources

PCR products of expected sizes of 905 bp for ble and 1281 bp for vgb were obtained from the transformants while in the wild-type sample, these DNA regions were absent (Fig.  2).

Perhaps most obvious among these aberrant features is petiolule length, (petiolule = stalk connecting leaflet to petiole in a compound leaf), which is distinctly elongated in both diploid and tetraploid E08 transformants while inconspicuous in wild type 'Hawaii 4'.

A predicted extra protein band of 46 kDa was observed in the transformants, while no such band was seen in the negative controls.

Overexpression of the A. niger ortholog of Pex11, which is known to be involved in peroxisomal proliferation [ 21] only resulted in minor peroxisomal proliferation in the A. niger transformants, while peroxicretion was not enhanced (figure 13).

Expression of an approximately 80/89 kDa protein band was seen in all the transformants while the corresponding parental strains failed to express the same.

For the ade2 assay, the numbers of Ade+ Ura+ transformants were compared to Ade− Ura+ transformants, while for the leu2 assay Leu+ Ura+ transformants were compared to the number of Leu− Ura+ transformants.

Thereby a 2900 bp fragment indicated successful deletion of phlp1 in the respective transformant while the presence of the wild type fragment (990 bp) revealed ectopic or no integration of the deletion cassette (data not shown).

The highest levels (up to 19·2 mg g−1 d. wt) accumulated in a progeny of a cross between a purple ANT1 and a IFS/ CHI transformant, while the second highest concentration was found in a plant derived from a selfed IFS2-12 transformant.

The pYES 2.1 TOPO TA is a E. coli- Yeast shuttle vector [ 16], propagated in E. coli using bacterial selection marker for Ampr; while the transformants in the yeast BY4741 strain background are selected for the URA3 marker.

Transgene integration was verified by mPCR in all primary transformants (T0), while immunoblot analysis confirmed that the protein HrpZPsph was produced and the signal peptide was properly processed.

Transformants varied in expression level.