Your English writing platform
Discover LudwigSuggestions(2)
Exact(1)
Subsequent to the choice of suitable expression vectors and proper host strains, and transformation of the expression cassettes, it is important to select for transformants which show high expression levels of the desired protein.
Similar(59)
We obtained 44 co-transformants, and identified co-transformant L 61, which showed the highest laccase activity among all the transformants.
We obtained 44 co-transformants, and identified co-transformant L 61, which showed the highest laccase activity among all of the screened transformants.
The transformant TYEGALLAC3-6, which showed the highest enzyme activity at 41.5 mU/ml culture supernatant, was selected for further study.
By combining the SNVs from independent transformants, regions can be observed in AID* and sA3G* genomes which show pronounced mutational peaks.
Which show?" "Nashville, last October".
Eight colonies were randomly analyzed by Southern blot, which showed that one of the Fas2 alleles had been replaced by SAT1 flipper cassette (Figure 1, lane 2) in each transformant.
The normalized growth rate of the entire group of 13 strains was 0.57+/−0.15 Table 3. The liquid test appears less sensitive than the plate test for transformants which had consistently shown SPI enrichment.
Based on an analysis of five generations of subcultures, PCR and Southern blotting assays, the Ptrpc-hph cassette had integrated into the genomes of all transformants, which contained single copy of the hph gene and showed mitotic stability.
DNA was sequenced from the transformants, which confirmed the presence of the suaA23 mutation.
Furthermore, the effect of this bgl2 mutation was reproduced in the common strain QM9414 in which the transformants showed cellulase production comparable to that of PC-3-7.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com