Expression of the wild type IRX7, IRX8 and IRX9 cDNAs under control of the pVND6 or pVND7 promoter in the respective irx7, irx8 and irx9 mutant backgrounds yielded improved growth patterns of both rosette size and stem height for all transformant lines, when compared to the respective irx mutant.
The bands from the transformants were faint or missing when compared to P. capsici strains SD33 and CK.
The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants.
Considering the haa1Δ transformants, the suppressors were able to rescue haa1Δ susceptibility phenotype by shortening the latency period by at least 20 h when compared with the haa1Δ strain transformed with the empty vector.
Mutation of the two potential glycosylation sites in PGU1 showed that the two residues individually (N318D, N330D) did not affect secreted enzyme activity, but the double mutant caused a 50% reduction in enzyme activity when compared to the wild-type PGU1 transformant.
In filamentous fungi such as Aspergillus niger, homologous recombination frequencies are extremely low when compared to the yeast Saccharomyces cerevisiae, which makes the generation of homologous transformants time-consuming.
Results confirm of 94 transformants the previously mentioned stability tests, as the better growth rate for these mutants were observed when compared to the R-control strain (Figs. 4, 5).
Replicative transformants carrying RIB1-mCherry plasmids showed 2.95 times more GCH II activity and 2.44 times more riboflavin production when compared to untransformed.
Indeed, the growth of the T-7 transformant harboring four altered copies of 23S rRNA was retarded compared to its 1974 parent (p < 0.01), even when compared to the 1974M1 mutant (p < 0.01).
