Exact(1)
The presence and correct orientation of the inserted promoter in plasmid extracted from transformants were validated by PCR using GFP_bdhA_F and GFP-R.
Similar(59)
The presence of the GFP sequence in the plasmid extracted from transformants was validated by PCR using the primers pXDC39-F and GFP-R.
The presence of bdhA in the plasmid extracted from transformants was validated by PCR using the primers PromF, which hybridizes to the Ptac promoter in pMMB207c, and Com_bdhA_R.
Only ten were validated.
Positive results were validated.
As mentioned above, many of these can be validated qualitatively, both by following the colony size of single transformants on solid media (Figure 3) and by monitoring the growth of pure cultures in liquid medium (Table 3).
Transformants were selected on SM-Ac (containing acetamide as the sole nitrogen source) and correct integration was validated with primer pairs 5/87 for pAGS7 and with 5/MRC1_r and 88/158 for pAGS8/pAGS8H.
"Now, it's been validated".
Women need to be validated.
"Everything has been validated.
Being validated.
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