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However, the transformants were unable to grow with either D-galacturonate or D-glucuronate as sole carbon sources (data not shown).
In contrast, YEpOLEX- Lca-SPVE and YEpOLEX- Lca-GATD transformants were unable to grow on media lacking UFAs.
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If the selection marker is lost, pyrF transformants are unable to metabolize 5-FOA, thereby preventing cell death, and uracil in the plates would facilitate cell growth.
Of 300 transformants tested, three were unable to cause necrotic lesions on detached Key lime leaves.
However, these transgenic lines were derived from the same transformant vector, which were unable to distinguish them from each other using common methods.
Because the mus81 Δ yen1 Δ diploid grows very slowly and has low transformation efficiency we were unable to generate sufficient Ura+ Ade+ transformants for meaningful analysis.
Surprisingly, however, and although we screened at least 100 primary transformants for each construct, we were unable to detect an EGFP signal in nonfunctional SUB cSUBmut:EGFP sub-1 plants (Figure 7M P).
After testing several thousands of transformants two clones were identified that were unable to grow when the sepF mutant allele was overexpressed by the addition of xylose to the growth medium.
Similarly, Mesak et al. (2009) cloned the V. harveyi frp into their constructs for use in S. aureus but were unable to obtain any transformants with these plasmids.
We were unable to obtain a transformant of D. melanogaster LHR-YFP into the same attP site, so as an alternative, we used P element transformation and obtained three independent transgene insertion lines.
Conversely, random control sequences that also contain ACS motifs (identity to the consensus 64 82%) were unable to promote replication (no transformants produced when these sequences were inserted in vectors pRS405 and pRS405-CEN (Figure 2, see also Figure S2D).
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