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The transformants were treated with MMS and cell extracts were prepared at different time points.
The transformants were treated with 0.01% MMS for 0, 1, 2, 3 and 4 h, and the survival rates were measured by the colony formation assay.
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One milliliter of whole mycelia extracts from the TU-6 or TU6-vgb+ transformants was treated with 20 mg of sodium sulfite.
Transformant B. choshinensis (pNCMO2-TFF1) cells were centrifuged and removed; the supernatant was treated with 60% ammonium sulfate to precipitate the secreted proteins.
After overnight incubation putative transformants were streaked on selective media.
The transformants were cultured and selected.
Transformants were selected on SD-URA plates.
Putative transformants were selected on kanamycin 100 mg/L.
Transformants were screened for growth in the absence of uracil.
Transformants were selected on plates with ampicillin and S-gal.
The plates with potential transformants were incubated at 30°C for at least 3 days until transformants appeared.
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