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Up to 95% of transformants were the result of homologous integration events [ 20].
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Based on these results, the transformants were approved for the quarantine field trials following the Type 1 Use Regulations.
The transformants were selected using the selective media supplemented with the antibiotic kanamycin (100 mg/L).
The transformants were selected using the selective media supplemented with the antibiotic kanamycin (100 g/L).
Several independent transformants were isolated, the characterization of one of them is shown.
In each experiment two independent transformants were analyzed, the average is shown.
The transformants were selected on the basis of green fluorescence, total genomic DNA (gDNA) was extracted from these fluorescent transformants.
The transformants were named as the yeast mixture with comprehensively mutated CBM.
Colony forming units (CFUs) of the transformants were detected in the presence of inducer.
The transformants were streaked on the selective medium for two successive rounds and tested by PCR.
The His+ transformants were screened for the best expression performances by prolongation of the ecarin clotting time.
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