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Six riboflavin independent transformants were tested for co-transformation of pTRcre2 via complementation of these creB1937 phenotypes.
The esa1 -414 temperature-sensitive strain was transformed with the library, and transformants were tested for growth at both permissive and restrictive temperatures.
To verify that the gene encoding siderophore trait is harbored on pSID/EGYII or not, these transformants were tested against E. amylovora strain EGY1 DSM 101800 using well-cut diffusion test.
The transformants were tested for their capacity to grow on uracil or 5FU containing media.
The transformants were tested for growth on M63 minimal medium including 10% (w/v) of glucose.
Notably, at least three independent transformants were tested for the survival plating to rule out clonogenic variation of the effects.
Similar(33)
The exoglucanase activity of the Thermotoga transformants was tested with MUC plates.
Co-transformants were tested for their ability to activate the histidine marker gene by assessing yeast growth on medium lacking leucine, tryptophan and histidine (-L-T-H) ability toeir activatetheactivate the LacZ reporter gene (X-Gal).
Transformants appearing were tested for their residual markers.
Plasmids obtained from the transformants and were tested by PCR for the presence of PcNLP gene insert.
Transformant lines were tested for IFM expression by crossing to UAS-GFP.
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