Sentence examples for transformants were targeted from inspiring English sources

On the other hand, 20 87% of stable transformants were targeted using an AAV-NANOG-targeting vector designed for the promoter-trap strategy.

At the HPRT1 locus, up to 1% of stable transformants were targeted via HR with an AAV-HPRT1 targeting vector, without loss of pluripotency.

Transformants were screened by PCR using target gene-specific and kanamycin gene-specific primers (Additional file 5).

Transformants were selected on 5-FOA plates and targeting was confirmed by PCR.

Multiple overexpression transformants were generated and PCR of the target genes were performed to confirm stable transformation.

The transformants were confirmed by locus-specific PCR using specific primer targeting a region immediately upstream of downstream of the homology region and a primer targeting the STn5 region.

The target gene alleles of these transformants were analyzed by PCR with verifying primer pairs (Table S3).

The target gene alleles of these transformants were analyzed by PCR with verifying primer pairs (Table S3 ). Isolation of gene deletion mutants by counter-selecting transformants with 5′-FOA.

Once pLRMG-orc1/whip transformants were purified such that the WT allele of the target gene could not be amplified by PCR in the cultures, the transformant cells were diluted and spread onto plates containing uracil and 5′-FOA.

DR- white.mu was also targeted to the 51C1 locus of chromosome 2 and stable transformants were selected based on y+ expression.

The transformants were selected for resistance to kanamycin and subsequently analyzed for the loss of target sequences in the ST556 chromosome by PCR amplification and DNA sequencing.